Journal: eLife
Article Title: Dysfunctional hippocampal-prefrontal network underlies a multidimensional neuropsychiatric phenotype following early-life seizure
doi: 10.7554/eLife.90997
Figure Lengend Snippet: ( A ) 3-D brain diagram and coronal sections representing the brain regions investigated. ( B–E ) Representative photomicrographs from immunohistochemistry each pair of columns represents a group comparison (left control (CTRL) and right ELS) and each line is related to CA1 and PL, respectively. ( B ) NeuN immunohistochemistry showed that ELS rats did not present neuronal loss. Parvalbumin (PV) immunohistochemistry. ( D ) mGluR5: ( E ) GFAP, showing increased astrogliosis induced by ELS (Mixed-effects model). GL: Granule layer of dentate gyrus; CA1: Cornu Ammonis 1; EC: Entorhinal cortex; IL: Infralimbic cortex; PL: Prelimbic cortex; TRN: Thalamic Reticular Nucleus. *p<0.05. CTRL n=11, and ELS n=14. Error bars represent the mean ± SEM.
Article Snippet: We used the primary antibodies against NeuN (Chemicon, USA; 1:1000), PV (Calbiochem, USA; 1:500), mGluR5 (Millipore, USA; 1:200), and GFAP (Dako, Denmark; 1:500) proteins, and biotinylated secondary antibodies (Dako, Denmark; 1:200).
Techniques: Immunohistochemistry, Comparison, Control